Prices
Routine sequencing
Full length amplicon sequencing - 15 EUR*
For amplicons starting from 100 bp
16S / ITS sequencing - from 35 to 150 EUR*
price depends on number of reads
Whole plasmid sequencing - 15 EUR*
For plasmids from 1,5 to 30 kb.
dsDNA bacteriophage genome sequencing - 150 EUR*
Phage Direct - 160 EUR*
100 €/sample for ≥10 phages
*prices do not include VAT
Small genome sequencing - 100 EUR*
For bacterial, fungal genomes up to 12 Mb
Additional bioinformatics analysis - 50 EUR/hour*
Upon request
RNA Sequencing (direct RNA or cDNA sequencing)
Upon request
110 €/sample for ≥10 phages
Custom sequencing projects
The price depends on the number of samples. Bioinformatic analysis is not included.
Services
Whole plasmid sequencing
Full length amplicon sequencing
Long read whole plasmid sequencing with automatic assembly, annotations, variant calling.
Long read PCR amplicon sequencing with automatic assembly, annotations and variant calling.
Fast and highly accurate whole plasmid sequencing for plasmids from 1,5 kb to 30 kb;
No primers;
No prior information about the plasmid;
Ideal to resolve plasmid recombination and duplications.
Fast and highly accurate sequencing for all amplicons starting at
100 bp;
No primers;
No prior information about the amplicon;
Reads are not fragmented and fully capture the sequence resolving variants.
Sample requirements:
15 µl of 40 ng/ul purified plasmid DNA.
Sample requirements:
10 µl of 30-40 ng/ul purified PCR amplicon >5kb in length.
10 µl of 10-20 ng/ul purified PCR amplicon, >1kb <5kb in length.
10 µl of 5 ng/ul purified PCR amplicon, >200 bp <1kb in length.
10 µl of 1 ng/ul, for amplicons <200 bp.
We highly recommend using column purification method and eluting the plasmid with water or a buffer without EDTA.
We highly recommend using column purification method and eluting the plasmid with water or a buffer without EDTA.
Small genome sequencing
Bacteriophage genome sequencing
Long read sequencing of bacterial or fungal genome up to 12 Mb with an automatic assembly and annotation.
Fast and highly accurate genome sequencing;
Chromosomes and plasmids resolved;
Automatic genome annotation;
Sample requirements:
20 µl of 40 ng/ul high purity, high molecular weight genomic DNA.
We highly recommend using column purification method and eluting the plasmid with water or a buffer without EDTA.
Long read sequencing of dsDNA bacteriophage genome up to 250 Kb with automatic assembly and annotation.
Fast and highly accurate genome sequencing;
PCR based library preparation;
Automatic assembly and genome annotation;
Sample requirements:
10 µl of 10 ng/ul high purity, high molecular weight bacteriophage genomic DNA.
We highly recommend using column purification method and eluting the plasmid with water or a buffer without EDTA.
Custom sequencing projects
Accurate long-read sequencing of reverse transcribed and PCR amplified RNA sample.
Service options:
Direct RNA sequencing - native RNA sequencing (without PCR amplification), using the ONT PromethION. Ideal for analysing RNA modifications, gene and isoform expression with minimal bias;
Option to multiplex different samples during the same run. Throughput: up to 24 samples per chip.
Please contact us at orders@seqvision.com with your specific needs.
Phage Direct sequencing
Oxford Nanopore based sequencing of DNA suspension with automatic assembly and annotation.
Up to 500 kb genome size;
Quickest phage sequencing to data;
DIRECT sequencing from phage suspension;
No DNA purification on your side;
Ideal for screening and isolating new phages;
Ideal for analysing phage mutants.
Sample requirements:
30-50 µl of high titer phage suspension (>109 pfu/ml)
Phage suspension should not contain any bacteria or cell debris.
16S sequencing
Long read sequencing of the full 16S gene region with automatic analysis.
No fragmentation;
PCR included;
Full report;
Sample purification option.
Sample requirements:
10 µl of 10 ng/ul high purity, DNA sample.
We recommend eluting in water or a buffer without EDTA.
ITS sequencing
Accurate long-read sequencing of RNA samples, with option to detect RNA modifications.
No fragmentation;
PCR included;
Full report;
Sample purification option.
Sample requirements:
5-100 ng of polyA enriched mRNA in 10 ul of DNA-free water.
1-2 ug of total RNA in 20 µl of DNA-free water.
5-100 ug any type of RNA (we will add polyA required for sequencing).
We recommend eluting in water or a buffer without EDTA.
Direct RNA sequencing
Long read sequencing of the full ITS1-ITS4 region with automatic analysis.
cDNA sequencing
We offer other DNA and direct RNA sequencing solutions not limited by small standardized services.
The services include:
Whole genome sequencing of large eukaryotic genomes.
Transcriptomics: cDNA and direct RNA sequencing.
Metagenomic DNA sequencing.
DNA and RNA modification sequencing.
Service options:
cDNA sequencing using the ONT PromethION. Ideal for analysing any type of RNA for gene and isoform expression.
Suitable even for low concentration RNA samples.
Option to multiplex different samples during the same run. Throughput: up to 24 samples per chip.
Sample requirements:
10 µl of 10 ng/ul high purity, DNA sample.
Output: ~50-100 Gb of data per PromethION P2 run.
Sample requirements:
100-200 ng of polyA enriched mRNA in 10 ul of DNA-free water.
10 ug of total RNA in 20 µl of DNA-free water.
100 ng - 1 ug any type of RNA (we will add polyA required for sequencing).
Output: ~15-25 Gb of data per PromethION P2 run. Throughput: up to 24 samples per chip.
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